Megraft: A software package to graft ribosomal small subunit (16S/18S) fragments onto full-length sequences for accurate species richness and sequencing depth analysis in pyrosequencing-length metagenomes.
Johan Bengtsson, Martin Hartmann, Martin Unterseher, Parag Vaishampayan, Kessy Abarenkov, Lisa Durso, Elisabeth M. Bik, James R. Garey, K. Martin Eriksson, R. Henrik Nilsson
Published in Research in Microbiology. Volume 163, Issues 6–7 (2012), 407–412, doi: 10.1016/j.resmic.2012.07.001.

Download the Megraft package (version 1.0.2)

Read the manual

Please e-mail me if you encounter bugs or have questions! My e-mail address is firstname.lastname[a]microbiology.se (and my name is Johan Bengtsson).

Version history

  • 25th January 2013 – 1.0.2 – better handling of sequences with N bases in them.
  • 26th May 2012 – 1.0.1 – no code changes, Megraft has left beta stage, updated manual.
  • 30th March 2012 — 1.0.1 beta – slight changes in response to review comments, updated manual.
  • 16th December 2011 – 1.0 beta – first public release.

If you for any reason would be interested in an older version of Megraft, please e-mail me at the address below.

Contact information

Johan Bengtsson-Palme (firstname.lastname [at] microbiology.se)
University of Gothenburg
Department of Neuroscience and Physiology
Medicinaregatan 11
Box 434
405 30 Gothenburg