I am on a roll pushing out new software these days, an here’s the latest addition. This version of ITSx was finished up last month and seems to be stable enough for consumption by the users. Version 1.0.5 adds a new option: “
--anchor” which enables extraction of regions flanking the ITS sequences (and the 5.8S, LSU and SSU, if desired). The option allows for extraction of a number of bases at each end, e.g. “
--anchor 30” to get 30 bp before and after each ITS region, or all bases matching the corresponding HMM, by specifying “
--anchor HMM“. The update can be downloaded here.